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Monthly Archives: April 2012
And back to the lab!
After 8 weeks of annotating our dear little Manatee’s genome and working incessantly on our computers we finally get to welcome back our beloved wet lab! Don’t get me wrong, I thought annotating the genome was an amazing experience and … Continue reading
Posted in From the Phage Hunters
Tagged annotation, Manatee, PCR, phage cluster, subcluster
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Phorever Phage Hunters
Although this semester of Phage Lab we had to deviate from the exciting and hands-on aspect of phage hunting, we still learned so much about another aspect of phage research. The purpose of the first half of this semester was … Continue reading
How to Annotate a Genome
Second semester in Phage Hunting has been completely different from the fall. Last semester, we were in a standard biology lab every class, performing titer assays and streaks to isolate our own unique phage. I became the proud parent of … Continue reading
There and Back Again: A Phage’s Tale
by Eleni Padden On my very first day of college, I shuffled timidly into a lab room with absolutely no idea what I was getting myself into. As I glanced around and tried to size up my fellow classmates, I … Continue reading
Posted in From the Phage Hunters
Tagged annotation, inquisitive, Lord Byron, phage, scientist
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Dear Brutus,
by Andrea Carrassi It is my first semester in Phage Hunting, and I am already loving the challenges that we are faced with as a group. I have become fluent in terms such as HHPred, coding potential, BlastHits, Glimmer, Genemark, … Continue reading
Late to the Phage Party
By Skyler Uhl Unlike most of my fellow classmates, I have yet to isolate a phage of my own. I was not in Phage Hunting I last semester, so I was not able to join everyone else in their quest … Continue reading
Posted in From the Phage Hunters
Tagged annotation, bacteriophage, enrichment, genome, isolation, Manatee
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Bioinformatics…Round 2
You’d think that after months of searching for the best possible starts and stops of genes, blasting base pair sequences, and hhpred-ing protein sequences, two things would occur: 1) We’d know everything there is to know about Manatee’s genome and … Continue reading
Posted in From the Phage Hunters
Tagged annotate, bacteriophage, BLAST, genome, Manatee
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Design My Own Experiment…Say WHAT?!
After countless Blast hits, HHpred searches, SD scores, and Phamerator visits, the genome of Manatee has finally been completely annotated by the JHU Phage Hunters! Hold for applause… Now what do we do you may ask? Simply stated, we design … Continue reading
Posted in From the Phage Hunters
Tagged bacteriophate, design, experiment, experimental, phage
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