By Jinny Huang
Phage hunting is by far the most interesting class I have this semester. It requires hands-on work rather than sitting in a small classroom or huge lecture hall for an hour trying not to fall asleep. It is also much different from high school labs that I took, where the directions are super vague and they make you try to puzzle out the lab as you do it. Also, the lab notebook guidelines are much more relaxed, and I no longer have to write all my data in pencil and then retrace it in pen in order to not get points taken off for inaccurate data. I can proudly say this is the only class I’ve never skipped once the whole semester.
I actually started one class period later than the rest of my peers, so for the first few classes I was behind. I managed to catch up during the streaking portion, because my phage was quite easy to isolate so I didn’t have to streak as many times as other people.
So far, my phage isolation process has been going pretty well. I managed to isolate one distinct looking phage, calculate my MTL and HTL, and start the DNA prep and EM. I just analyzed my DNA prep today and it seems as though my DNA is pure and I have a decent concentration. I was actually pleasantly surprised by my DNA analysis results because I accidentally messed up a few of the steps of the DNA prep. I also started the electron microscopy today; however, the centrifuge was broken so I have to wait until next time to finish it.